Protective effect of chrysin on ischemia-reperfusion injury in rats and the relation with oxidative stress and excessive autophagy

Abstract

To explore the protective effect of chrysin on cerebral ischemia-reperfusion injury (CIRI) in rats and mechanisms. Fifty-four rats were divided into sham-operated, model and treatment groups. The treatment group was treated with 100 mg/kg chrysin for five days. Then, the CIRI model was established by middle cerebral artery occlusion in model and treatment groups. After modeling, compared with model group, in treatment group the neurological deficit score, brain water content and brain infarction percentage were decreased, the brain tissue superoxide dismutase level was increased, the malondialdehyde level was decreased, the nuclear factor-erythroid 2 related factor 2 and heme oxygenase-1 protein expression levels were increased, and the LC3-II and Beclin-1 protein levels were decreased (all p<0.05). The chrysin pretreatment may alleviate the CIRI in rats by reduction of brain tissue oxidative stress and inhibition of excessive autophagy, the significant causes of morbidity and mortality in neurological disorders.